10/15/10 LECTURE
Life Sciences 3 with Johnson at University of California - Los Angeles
About this deck
By: Olivia Akubuilo
Created: 2010-10-19
Size: 18 flashcards
Views: 29
Created: 2010-10-19
Size: 18 flashcards
Views: 29
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What is the function of Beta and Beta' on the core polymerase?
Both are required for DNA binding and catalysis
What is the function of the two alpha subunits on the core polymerase?
The alpha units are required for DNA binding and assembly.
What function does the omega subunit served on the core polymerase?
It stabilizes the Beta' binding.
The addition of the sigma factor makes a holoenzyme or the enzyme complete. What is its function?
- The sigma factor tells the polymerase where to bind in order to start transcription
- recognizes specific DNA sequences and there are multiple sigma factors in cells that recognize different sequences (in prokaryotes)
What are the three steps that transcription is divided into?
- Initiation
- Elongation
- Termination
In initiation which position describes where the transcription is starting.
At the +1 position is where transcription starts, and transcription carries on until we reach a signal that tells us to stop
- RNA polymerase is recognizing specific region in DNA promoter whcih promotes transcription
What is the difference between prokaryotes and eukaryotes when it comes to transcription and translation?
Transcription and translation occur simultaneously in prokaryotes because there is no nucleus
What is the coding region in a GENE which is DNA required to direct synthesis of an RNA or protein?
A coding region is important in translation and is considered downstream because it has positive base pairs.
- Coding region is always in positive territory
What is the function of the 5' untranslated region and the 3'untranslated region?
They signal both translation and transcription to start and stop.
What do promoters determine in regards to transcription initiation?
Promoters determine the sites of transcription initiation.
What is the difference between convergent and divergent promoters?
Convergent promoters have the polymerase run int one another and may stop before hand.
Divergent promoters have the polymerase moving away from each other
Divergent promoters have the polymerase moving away from each other
Simple promoters are recognized by SIGMA 70 and have a consensus sequence for both the -35 sequence and the -10 sequence what are these sequences?
- For -35: TTGACa (where a is conserved and has more variation)
- For -10: TAtAaT it has two positions that are less conserved
- This increases the frequency of transcription and the closer you are to the match is less conserved
In the sigma 70 promoter , there are some strong promoters one is the up-element, what does it do the rate of transcription?
It increases transcription and makes a site that interacts with alpha subunits of polymerase and makes it bind more often at site.
Some promoters lack the -35 sequence what does it need in order for transcription to proceed?
It may require the transcription factor to help polymerase recognize promoter and start transcription.
The Sigma 70 component has three sites of binding activity sigma and sigma2, and sigma4, which molecule does the alpha-CTD interact with? Also which molecules do the sigma2 and sigma4 bind?
The Alpha CTD interacts with the up-element promoters that interact with up element have three sites and interacts with polymerase.
- Sigma4 binds/recognizes the sequence at -35 and sigma2 binds/recognizes the sequence at -10.
In divergent promoters what sets up the the direction of the promoters to go opposite ways?
The polymerase binds promoters facing its direction and will go left to right on the -35 sequence and the -10 sequence.
- The bottom strand is used as the template
What is sigmaS function?
Stress regulator genes that only turns on when under stress.
What sigma32 do? and sigmaE, and 28 do as well.
- sigma32-It has heat shock genes
- heat up bacteria to high denaturation.
- sigmaE- Genes for envelope integrity
- gigma28- Flagellum assembly and chemotaxis
About this deck
By: Olivia Akubuilo
Created: 2010-10-19
Size: 18 flashcards
Views: 29
Created: 2010-10-19
Size: 18 flashcards
Views: 29
About StudyBlue
STUDYBLUE makes things that make you better at school.
Things like online flashcards with photos and audio.
Things like personalized quizzes and friendly reminders about when (and what) to study next.
Think of it as a digital backpack™: access to all of your study materials online and on your phone.
STUDYBLUE exists to make studying efficient and effective for every student, for free. Join us.
“I have used this website for three exams, and I see a huge difference in my test results.”
Naj
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