Writing Assignment 1- Summary of a Research Paper Write a paper, around 3-4 pages (typed, double-spaced, 12-point font) summarizing how and why the genetic manipulations were carried out in the research article cited below. The Paper: Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Raphael H. Valdivia, Alexander E. Hromockyj, Denise Monack, Lalita Ramakrishnan and Stanley Falkow, Gene, 173 (1996) 47-52 Why the cloning was done (5 points)- You can think of this in terms of both the immediate use (e.g., to see if GFP can be visualized in intracellular pathogens) and the bigger picture (e.g., developing tools to study how parasites infect cells). How the cloning was done (10 points)- This part will be technical and require that you read the paper closely. It should cover both the general strategy (e.g., PCR, restrictions digests and ligase to clone gfp into different vectors), and the specific (how DNA was isolated, how primers were chosen, what enzymes were employed, etc.). All of that detail is in the paper, but you should not simply copy it over into your paper. If there are figures in the paper detailing the cloning, you may refer to them but must still provide a prose summary. Accuracy and understanding are what you should demonstrate. Overall Summary of Findings (5 points)- This follows from the ?Why? section?how did it turn out? Was the hypothesis verified (or ruled out)? What did they learn; what happens next? Were there any limitations or shortcomings in the research? Style and Mechanics (10 points)- Is your paper well-written and free of grammatical and spelling errors? Does it flow easily? Is it well organized & clear? Due Date- The summary paper is due in lab during the week of November 2. Reminder- all work and expression should be your own. A summary is an exercise in reading, understanding, and paraphrasing. Your paper should be about cutting and pasting (i.e., cloning), not an example of it (i.e., plagiarism). If you?re not sure what?s fair, ask your GSI. Your GSI may add more specific requirements than those spelled out here. pBR322 vector pMV261 vector Marc Ammerlaan 173f09paper1
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