Bacteriologic / immunologgic methods

Biological Sciences 315 with Burton at State University of New York - Stony Brook

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By: charles braun
Created: 2011-04-05
Size: 32 flashcards
Views: 20

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Blood cultures

done when sepsis, endocarditis, meningitis, penuemonia or osteomelitis is suspected

blood cultures organisms most often isolated

gram + (cocci) staph. aureus, strep pneumoniae

gram - (rods) E. coli, K. pneumoniae, P Aeruginosa

what to check for in blood cultures

mixed w/ rich medium & anerobic growth

Growth in blood cultures

gram stain, antibiotic sensitivity

Throat cultures

test for B- hmolytic streptococci

throat culture

test with bacitracin- if growth inhibited prob group A streptococcus---> pyogenes

sputum cultures

performed when pneumonia, TB, or lung abscess suspected

cause of pneumonia in hospital

staph aureus, K. pneumoniae ,or other gram - rods

cause of pneumonia outside hospital

strep. pneumonia gram + diplococcus

Sputum staining

if TB suspected, acid fast stain is done

spinal fluid cultures

performed when meningitis is suspected

acute bacterial meningitis

usually caused by N. meningitis, step pneumoniae or H. influenze

acute staining

gram - stained followed by immunological tests

subacute

usually caused by M. tuberculosis and fugus crytococccus

Subacute staining

spinal fluid acid fast stained and cultured

stool cultures

used in cases of enterocolitis. usually cases of culpritis are shigella, salmonella, and camplyobacter

stool cultures gram staining

usually not useful because of large bacteria flora in colon

Stool staining

methly blue stain if large number of leukocytes present

genital tract cultures- male

presence of garm - diplococci within neutrophils---> N. gonorrhea

genital tract cultures- female

chlamydia trachomatis

syphilis

cultured in human cells or chickin eggs. motile spirochetes

quellung reaction

swells when specific antibody binds

slide agglutination test

specific antibodies against cell wall are mixed with cells on microscope slide. form large clumps

macConkey Agar or EMB agar

Inhibit gram +

urine cultures

most common case is E. coli, others are enterobacter, proteus, strep faecalis

asynptomatic patients

at least 100,000 cells/mL

symptomatic patients

need at least 100 cells/mL

ELISA

anitgens detected w/ specific antibody that has enzyme attached at Fc end

Serological techniques

isolate blood serum containing antibodies of unknown specifity and react with antibodies against known organisms

PCR

use ssDNA primers to amplify gene from organism's genome

Hybridization

hydridize ssDNA with probe attached to organism's DNA

Nucleic acid sequencing

determinine sequence of DNA or RNA from organism

About this deck

By: charles braun
Created: 2011-04-05
Size: 32 flashcards
Views: 20

About StudyBlue

“I have used this website for three exams, and I see a huge difference in my test results.”
Naj