Chapter 7: Practice Questions
Biology 240 with Hughes at University of Tennessee - Knoxville
About this deck
By: Terry Nowell
Textbook:
An Introduction to Genetic Analysis (INTRODUCTION TO GENETIC ANALYSIS (GRIFFITHS))
Introduction to Genetic Analysis
Introduction to Genetic Analysis & Mega Solutions Manual
Introduction to Genetic Analysis (Introduction to Genetic Analysis (Griffiths))
Created: 2011-11-08
Size: 56 flashcards
Views: 45
Textbook:
An Introduction to Genetic Analysis (INTRODUCTION TO GENETIC ANALYSIS (GRIFFITHS)) Introduction to Genetic Analysis Introduction to Genetic Analysis & Mega Solutions Manual Introduction to Genetic Analysis (Introduction to Genetic Analysis (Griffiths))Created: 2011-11-08
Size: 56 flashcards
Views: 45
About StudyBlue
STUDYBLUE makes things that make you better at school.
Things like online flashcards with photos and audio.
Things like personalized quizzes and friendly reminders about when (and what) to study next.
Think of it as a digital backpack™: access to all of your study materials online and on your phone.
STUDYBLUE exists to make studying efficient and effective for every student, for free. Join us.
“Simply amazing. The flash cards are smooth, there are many different types of studying tools, and there is a great search engine. I praise you on the awesomeness.”
Dennis
Dennis
Sign up (free) to study this.
EXAM 3
Units 10, 11
The flow of information from gene to phenotype is:
DNA --> mRNA --> protein --> metabolic pathways
D.
In Griffith's experiment (infecting mice with Streptocoocus pneumoniae) he used two strains of bacteria, a "smooth" virulent strain with a polysaccharide coat and a non-virulent "rough" strain without a polysaccharide coat. After which of the treatments below did the mice survive?
Mice revieving dead smooth bacteria
B.
In the 1940s, most scientists believed that the genetic material had to be protien. If this prevailing opinion had been right, what would Avery et al. have found in their experiments?
Protease, but not DNAse or RNAse would have destroyed transformation of rough bacteria
B.
Hershey and Chase carried out an experiment to determine if DNA was also genetic material of bacterial viruses. Which of the following is not part of their experiments?
32P was found associated with phage coats
D.
Tobacco mosaic virus consists of a protein coat surrounding RNA. A TMV strainA virus was stripped of its coat and a protein coat from strainB was reconstructed over the strainA RNA core. If this new hybrid virus is used to infect a tobacco leaf, which coat will be produced in the progeny viruses?
Coat protein A
A.
In the experiment involving hybrid tobacco mosaic viruses described above, what did this experiment demonstrate?
RNA is the genetic material for this virus.
B.
What is the name for a small RNA (approximately 70bp) that carries an amino acid to the ribosomes?
transfer RNA
B.
Chloroplasts and mitochondria have circular chromosomes? They also have ribosomes. Are the ribosomes eukaryotic or prokaryotic in structure?
Prokaryotic
B
If the anti codon is 3' UAC 5', what is the codon?
5'AUG3'
C.
What direction is the arrow number one in the image to the right?
5' to 3'
A.
What enzyme is responsible for relaxing supercoiled DNA during replication?
Topoisomerase
C.
Replication of DNA cannot be a continuous process because:
replication proceeds only in the 5'-3' direction of the new strand
B.
DNA replication in prokaryotes begins with -
Binding of DNA A proteins adjacent to the origin of replication
D.
Telomerase functions to:
add DNA to the ends of the chromosomes
D.
DNA labeled with 15N and centrifuged in a cesium chloride gradient will form a band ---
Below DNA containing 14N
B.
(2X)
A DNA strand is labeled with a heavy nitrogen (15N). If the conservative model of replication had been correct, what would you see after two rounds of DNA replication in medium containing 14N?
one heavy, one light band
A.
Are there any known exceptions to the rule that DNA replication is semiconservative?
No
E.
What makes a primer for DNA polymerase during DNA replication?
Ligase
E.
Which DNA polymerase removes RNA primers following DNA synthesis?
Polymerase I
A.
(2X)
After removal of the RNA primers, Okazaki fragments are joined through a reaction that involves which enzyme?
DNA ligase
C.
PRACTICE QUIZ QUESTIONS
Unit 10
What hypothesis was Griffith testing when he discovered "transformation"?
The smooth IIIS form of Streptococcus causes pneumonia
B
Why did Avery et al. treat bacterial extracts with proteinase?
If protein was the genetic material, proteinase would eliminate transforming activity
A.
In the Hershey-Chase experiment, 32P was chosen as a label because -
It labels DNA
A.
A nucelotide consists of
A sugar, a base, and a phosphate
A.
Which of the following is a purine?
Adenine
B.
A purine with an amine (NH2) group on the 6th carbon is
Adenine
A.
A five carbon sugar lacking an oxygen at the Number 2 Carbon is found in -
DNA
A.
In DNA, guanine pairs with --
Cytosine
B.
In RNA, uracil pairs with --
Adenine
A
Which of the following will require a higher temperature to denature?
DNA with a high G-C content
A.
What is a function of messenger RNA?
It is a direct copy of a gene
C.
The genetic material of retroviruses such as HIV is
RNA
B.
Retroviruses replicate via a ___________ intermediate
DNA
B.
Prokaryotic chromosomes are typically -
circular
B.
Proteins complexed with DNA include --
all of the above are correct
D.
A. non histone proteins
B. histone proteins
C. regulatory proteins
Which of the following is not a key property of hereditary material?
it must be able to adapt itself to each of the body's tissues
D.
If thymine makes up 15% of the bases in a specific DNA molecule, what is the percentage of the base adenine?
15%
A
If thymine makes up 15% of the bases in a specific DNA molecule, what is the percentage of the base guanine?
35%
C.
PRACTICE QUIZ QUESTIONS
Unit 11
Messelsen and Stahl designed an experiment to test different theories of DNA replication. One of the theories stated that the original DNA strand was retained intact and used as a template for a new strand. This model of replication was called ---
Conservative replication
A.
After one replication in 15N, how many bands should be observed in a Cesium Chloride gradient?
One
A.
After two rounds of replication, what bands will be onserved in a cesium chloride gradient?
One medium and one heavy band
D.
Taylor, Woods and Hughes labeled Visua DNA by allowing new DNA synthesis in the presence of radioactive thymine. After DNA replication (S phase of the cell cylce), they should have observed ---
Both chromatids of a chromosome were labeled
B
DNA replication begins at an origin of replication. In bacteria, there is a single origin of replication. How many origins of replication are found in the much larger genomes of eukaryotes such as mammals?
Thousands and tens of thousands.
C.
The enzyme DNA polymerase synthesizes new DNA in the --
5'-3' direction
A
The DNA polymerase in bacteria which is responsible for DNA synthesis is -
Polymerase III
C.
DNA repair is an important function of cells. Which polymerase is primarily active in repair?
Polymerase II
B.
Protiens which help open the double helix for DNA synthesis are --
DNA helicases
A
Proteins which release supercoiling that results from opening in a replication bubble are --
DNA topoisomerase
D
The enzyme which sealse the DNA backbone on the discontinuous strand is -
Ligase
C
An RNA primer is synthesized by -
Primase
E
DNA polymerase requires a primer to synthesize DNA
True
Without telomerase, what would happen to the ends of chromosomes after each round of replication?
Chromosomes would become progressively shorter
Acronym for HSADRO
Helices unwind DNA
Single stranded binding proteins hold DNA open
An RNA primer is synthesized
DNA polymerase synthesizes DNA in the 5'-3' direction on both strands
RNA primers are removed and the gap is filled
Okazaki fragments are joined by ligase
About this deck
By: Terry Nowell
Textbook: An Introduction to Genetic Analysis (INTRODUCTION TO GENETIC ANALYSIS (GRIFFITHS))
Introduction to Genetic Analysis
Introduction to Genetic Analysis & Mega Solutions Manual
Introduction to Genetic Analysis (Introduction to Genetic Analysis (Griffiths))
Created: 2011-11-08
Size: 56 flashcards
Views: 45
Textbook:
An Introduction to Genetic Analysis (INTRODUCTION TO GENETIC ANALYSIS (GRIFFITHS)) Introduction to Genetic Analysis Introduction to Genetic Analysis & Mega Solutions Manual Introduction to Genetic Analysis (Introduction to Genetic Analysis (Griffiths))Created: 2011-11-08
Size: 56 flashcards
Views: 45
About StudyBlue
STUDYBLUE makes things that make you better at school.
Things like online flashcards with photos and audio.
Things like personalized quizzes and friendly reminders about when (and what) to study next.
Think of it as a digital backpack™: access to all of your study materials online and on your phone.
STUDYBLUE exists to make studying efficient and effective for every student, for free. Join us.
“Simply amazing. The flash cards are smooth, there are many different types of studying tools, and there is a great search engine. I praise you on the awesomeness.”
Dennis
Dennis