Lecture 37
Biochemistry 401 with Foley at Michigan State University
About this deck
By: Kiandra arnold
Textbook: Biochemistry (Looseleaf)
Created: 2010-08-07
Size: 30 flashcards
Views: 18
Textbook: Biochemistry (Looseleaf)
Created: 2010-08-07
Size: 30 flashcards
Views: 18
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Different Times(Temperospatial regulation)
- Different developmental states
- Different enviromental cues
Different Spaces( Temperospatial regulation)
- Different tissue types
- Different cell types
Differences in genome organization(transcriptional regulation)
- Chromosome struture
- Gene organization
Differences in RNA polymerase(Transcriptional regulation)
- Targeting
- Structure
Differences in organism(transcriptional regulation)
- Cell/body organization
- Environment/growth rate
Cis Factors
- DNA sequences: Promoters , enhancers, silencers
- Sliencer and enchancer elements
- Cis regulatory elements outside promoter region
- Sites for trancription factor binding
Trans factors
- Proteins that regulate transcription
Repressors bind
- Silencers elements
Activators bind
- Enhancers elements
Structural Transcription Factor
- Minor Groove
- Major Groove
Chemical Transcription Factor
- Have Residues that can make the right contact with DNA in that region
DNA Binding Domain(DBD)
- Helix turn Helix,B-zip, Zinc Finger
Effector Domains(Activation or Repressor)
- Recruit other proteins
- Interact with polymerase II
- Interact with mediator proteins that bridge to poly II
True or False: In helix turn helix, one helix recognizes bases in the major groove and the second helix packs the first helix in place,making interactions with the phosphate backbone
- True
_____________ is the major motif for DNA binding domain in prokaryotes,recognization helix packs into major groove and second helix stabilizes this association
- Helix turn helix
Homeodomains
- Bind as homo or hetero dimers through HTH in major groove(eukaryotic)
B-zip
- Basic region insert into major groove
- Leucine Zipper: Dimerizaion-Coiled-coil interactions
- Stabilized by Leu in dimer interface
Zinc Finger
- Cys2His2 zinc finger wraps around DNA in the major groove
Zinc Finger Domain
- Zinc ions coordinated by 2 histidines and 2 cysteine residues
- Other Zinc residues can be coordinated by 4 cysteines
- Some transctiption factors have seven repeated zinc fingers,others like gluccorticoid receptor have only two
Prokaryotic Model: The Lac Operon
True or False: Prokarytoic genes necessary for common pathway are often organized in blocks called operons
- True
lac repressor
- Lac operon gene that down regulates transcripton of z,y,a
z,y, and a gene products
- Lac operon gene which is involved in lactose utilization as a food source
________________ bound to operator site prevents transcription of Z,Y, and A
- Repressor
T or F: Repressor inducer complex binds DNA
- False , it does not bind DNA
- Inducer binds to lac and reduces its affinity for operator
T or F: Decrease in glucose, results in increase for cAMP, which binds CAP,increasing affinity of CAP for the promoter
- True
T or F: Presence of lactose in the enviroment. Allolactose binds lac repressor ,reducing its affinity for the operator sequence. The operon is no longer repressed,but transcription is low. The operon is till not activated
- True
Tamoxifen
- Antagonists in some tissues
- Contain co-activators
- Prevents activation-decreasing transcription of ERE containing genes(EREs)
____________ is an agonist in other tissues tha that contain co-repressors and prevents repression , allowing transcription of ERE containing genes(bone tissue)-Osteoporosis therapy
- Tamoxifen
About this deck
By: Kiandra arnold
Textbook: Biochemistry (Looseleaf)
Created: 2010-08-07
Size: 30 flashcards
Views: 18
Textbook: Biochemistry (Looseleaf)
Created: 2010-08-07
Size: 30 flashcards
Views: 18
About StudyBlue
STUDYBLUE makes things that make you better at school.
Things like online flashcards with photos and audio.
Things like personalized quizzes and friendly reminders about when (and what) to study next.
Think of it as a digital backpack™: access to all of your study materials online and on your phone.
STUDYBLUE exists to make studying efficient and effective for every student, for free. Join us.
“Simply amazing. The flash cards are smooth, there are many different types of studying tools, and there is a great search engine. I praise you on the awesomeness.”
Dennis
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