Lecture 5 handout.dpdf.pdf
Microbiology 411 with Kendall/traxler at University of Washington - Seattle Campus
About this note
By: Vladimir Stovbyr
Textbook:
Molecular Genetics of Bacteria (Snyder, Molecular Genetics of Bacteria)
Created: 2010-03-27
File Size: 3 page(s)
Views: 6
Textbook:
Molecular Genetics of Bacteria (Snyder, Molecular Genetics of Bacteria)Created: 2010-03-27
File Size: 3 page(s)
Views: 6
About StudyBlue
STUDYBLUE makes things that make you better at school.
Things like online flashcards with photos and audio.
Things like personalized quizzes and friendly reminders about when (and what) to study next.
Think of it as a digital backpack™: access to all of your study materials online and on your phone.
STUDYBLUE exists to make studying efficient and effective for every student, for free. Join us.
“I have been getting MUCH better grades on all my tests for school. Flash cards, notes, and quizzes are great on here. Thanks!”
Kathy
Kathy
Sign up (free) to study this.
Micro/Genome 411 Lecture 5 SPONTANEOUS MUTATION I. Mutation due to replication errors A. Review of DNA replication (again!) B. Understanding / calculating mutation frequencies C. DNA polymerase error repair functions 1. Base selection 2. Proofreading D. Speed vs fidelity and the redundancy of repair functions II. Mutational fingerprinting A. Base pair substitutions B. Determining mutational spectra of lacI 1. Isolating lacI deletion mutants (= mapping strains) 2. Isolating unknown lacI- mutants 3. Identifying lacI amber nonsense mutants a. Amber suppressor strains b. Identifying the specific amber mutation 4. Construction and use of a mutational spectrum Lecture 5: Spontaneous Mutation Micro/Genome 411 ? G A T C ? ? C T A G ? G A T C ? ? C C A G ? G A T C ? C T A G ? G G T C ? C C A G ? G A T C ? C T A G ? G A T C ? C T A G ? G G T C ? C T A G ? G A T C ? C T A G ? G G T C ? C C A G ? G A T C ? C T A G Frequency of replication error: Base selection:10 -5 Proofreading:10 -4 Mismatch repair:10 -1 Total10 -10 Lecture 5: Spontaneous Mutation Micro/Genome 411 Kendall Gray Microsoft Word - Lecture 5 handout.doc
About this note
By: Vladimir Stovbyr
Textbook: Molecular Genetics of Bacteria (Snyder, Molecular Genetics of Bacteria)
Created: 2010-03-27
File Size: 3 page(s)
Views: 6
Textbook:
Molecular Genetics of Bacteria (Snyder, Molecular Genetics of Bacteria)Created: 2010-03-27
File Size: 3 page(s)
Views: 6
About StudyBlue
STUDYBLUE makes things that make you better at school.
Things like online flashcards with photos and audio.
Things like personalized quizzes and friendly reminders about when (and what) to study next.
Think of it as a digital backpack™: access to all of your study materials online and on your phone.
STUDYBLUE exists to make studying efficient and effective for every student, for free. Join us.
“I have been getting MUCH better grades on all my tests for school. Flash cards, notes, and quizzes are great on here. Thanks!”
Kathy
Kathy