Test 2 Microbial Chem 2

Microbiology 319 with Aarthi Sundararajan at University of Tennessee - Knoxville

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By: Sara Knoll
Created: 2010-03-23
Size: 19 flashcards
Views: 25

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transition step

-pyruvate to acetyl coA
-requires input of coenzyme A
-liberates CO2
-commits cell to TCA cycle
-drives metabolism towards electron transport chain
-forms reducing power 2NADH + 2H+
-form 1 precursor metabolite
-occurs 2x because of 2 pyruvate produced in glycolysis

tca cycle:

-also called krebs or citric acid cycle
-used in aerobic and anaerobic
-8 step cycle that converts acetyl co A into oxaloacetate
-produces = 4CO2, 2 ATP, 6NADH + 6H+, 2 FADH2
-forms 3 precursor molecules

major functions of electron transport chain:

-reoxidation of reduced coenzymes (NADH and FADH2) critical for recycling
-generation of ATP via oxidative phosphorylation

electron motive force relies on what?

electron gradient (step wise reduction in potential energy)
-stepwise release of energy creates a proton motive force
-proton motive force used to drive ATP synthesis

where does electron transport chain take place?

PROKARY = cell membrane
EUKARY = mitochondria/chloroplasts

why does electron transport chain rely on inorganic terminal electron acceptor?

-allows all ETC components to be reoxidized to continue function
-for aerobes O2 is reduced to H2O

what are the major components of the ETC?

-flavoproteins and cytochromes (integral membrane complexes)
-ubiquinones/quinones (lipophilic electron carriers)
-cytochrome c as oxygen reducing enzyme
-ATPase

what is energy yield of ETC?

-3ATP for every NADH
-2 ATP for every FADH2

what is the energy summary of total metabolic pathways?

SLP:
-TCA = 2 ATP
-GLYCOLYSIS = 2 ATP
Oxidative Phosphorylation:
-2 NADH = 6 ATP (glycolysis reducing power)
-2 NADH = 6 ATP (transition step)
-6 NADH, 2 FADH2 =22 ATP (TCA cycle)
-

how much ATP derived from ONE GLUCOSE MOLECULE:

38 ATP

how do you test for cytochrome-c oxidase

-bacteria mixed with one drop of water on slide
-transferred to test strip
-Positive rxn = change color to blue/purple color

how do you test for presence of catalase?

-bacteria on slide combined with H2O2
-positive rxn = formation of bubbles

urea as a source of nitrogen:

-vertebrates consume protein, create urea as a waste product (excreted in urine)
-some bacteria can use urea as nitrogen source
-survival advantage for colonizing urinary tract, waste water, etc.

(NH2)2 + CO = ?

CO2 + 2NH4OH

urea agar slant:

contains urea, peptidase, dextrose
-phenol red = pH indicator to detect ammonia if urea is hydrolyzed

urea test results=

ORANGE = -
YELLOW = fementation (-) /acidic
MAGENTA =pH 8.5 (+) urea hydrolyzed

indole test:

use SIM agar deep
-use Kovacs reagent to top and if red color then (+)
-black = H2S
-motility = movement away from stab

methyl red and voges proskauer:

-use MRVP broth (buffered peptone-glucose)
-used to distinguish between 2 classes of fermentation (mixed acid and 2,3-butanediol)
-Methly red test = mixed acid (red color +)
-voges test = 2,3 butane. (rose color = +)

citrate test:

-use Simmon's Citrate agar
-test ability to use Citrate as sole C source
-must have citrate permease to TRANSPORT and then citrase to OXIDIZE once inside cell
-pH indicator bromthymol blue (+ = blue/- = green)

About this deck

By: Sara Knoll
Created: 2010-03-23
Size: 19 flashcards
Views: 25

About StudyBlue

“I have used this website for three exams, and I see a huge difference in my test results.”
Naj