Transcription Overview DNA template strand 5? 5? 3? 3? ACT GAT TTA ACT CGG TGA C__ ___ ___ ___ TRANSCRIPTION 5? 3? mRNA UGA ___ ___ ___ ___ 3 nucleotides (bases) = Codon = 1 amino acid (or start/stop) AUG UGU UUA CGA AUU Exon 1 Exon 2 INTRON 1 UAA Overview of mRNA Processing AUG UGU UUA CGA AUU UAA AAAAAA 5? cap Add 5? cap Splice out introns Add poly A+ tail Codons are universal, non-overlapping, and degenerate (more than 1 codon can specify the same amino acid). GGT Gly (G) GGC " GGA " GGG " GAT Asp (D) GAC " GAA Glu (E) GAG " GCT Ala (A) GCC " GCA " GCG " GTT Val (V) GTC " GTA " GTG " G AGT Ser (S) AGC " AGA Arg (R) AGG " AAT Asn (N) AAC " AAA Lys (K) AAG " ACT Thr (T) ACC " ACA " ACG " ATT Ile (I) ATC " ATA " ATG Met (M) A CGT Arg (R) CGC " CGA " CGG " CAT His (H) CAC " CAA Gln (Q) CAG " CCT Pro (P) CCC " CCA " CCG " CTT Leu (L) CTC " CTA " CTG " C TGT Cys (C) TGC TGA Ter TGG Trp (W) TAT Tyr (Y) TAC TAA Ter TAG Ter TCT Ser (S) TCC " TCA " TCG " TTT Phe (F) TTC " TTA Leu (L) TTG " T G A C T AUG UGU UUA CGA AUU UAA AAAAAA 5? cap mRNA TRANSLATION Met Cys Leu Arg Ile Stop rRNA: ribosomes tRNA: amino acids Polypeptide (protein) GUU UAC GAA UUA AAA TRANSLATION CYS VAL TYR GLU LEU LYS A UGU A What if reading frame shifts? TRANSLATION OVERVIEW
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