BioBM3320 Principles of Biochemistry Biochemistry and Molecular Biology of Nucleic Acids Professor Bik Tye TAs Adam Brady oab8 Patrice Ohouo pyo4 Jon Paczkowski jep268 Jessica Wayt jw657 Office hours Mon-Fri Course website http://blackboard.cornell.edu/ and then log in with your user ID Textbook: Lehninger 4th or 5th Edition by David Nelson and Michael Cox Course packet available at Campus bookstore at $13.75. Lectures can be downloaded from the web. 1st page - timetable for Syllabus is only approximate and subject to changes. Assignments from back of chapter and study guide after every lecture. Best problems for this course are old exams. Grading - based on three in class prelims (Thurs, 2/18, 3/18, 4/22 in PLS233 and RRB125) and a final (5/?) 3 prelims will count for 70% of the grade, final 30% or 2 prelims will count for 60%, final 40%, whichever gives you the better score No make-up prelims. If a prelim is missed, 2 prelims count for 60% of the grade, final 40%. Mean grade will be curved to around a B+ Reviews: Mondays (4:30 - 6:00 PM) WRN245 Adam and Patrice Wednesdays (5:00 - 6:30 PM) WRN131 Jessica and John Pre-submisssion of questions or topics for review by email is encouraged. Review of problem sets. Overview Biosynthesis of macromolecules DNA, RNA and Protein Information pathways DNA RNA Protein A,T,G,C A,U,G,C 20 amino acids New Era in Biology - sequencing of entire genomes Genomics Studies of single gene or enzyme give rise to the studies of all genes in the genome and all proteins inside a cell. New disciplines based on genomics Systems biology Comparative genomics - comparative studies of genomes Population genetics Evolutionary genetics Anthropology (human migration) New applications Advances in Medical Research Mapping disease genes (using SNPs) Microarrays for diagnostics Review of concepts and material that you are expected to know for this course The coding capacity of the genome is enormous ! 48 424 Triplet code 2.8 x 1014 Number of combinations for a word of 8 letters Coding capacity Vocabulary Organization and Structure of Cells Prokaryotes versus Eukaryotes Prokaryotes as models for eukaryotes ?What?s true for E. coli is true for elephants? - Monod Bacterial Cell A single (plasma) membrane no nucleus or organelles much larger in size than prokaryotes 103-104 times larger! Compartmentalization of chromosomes, transcription and protein synthesizing machineries Information transfer - Prok and euk use the same principles but differ in details. Most differences due to the presence or absence of a nucleus. DNA replication, transcription and translation all take place in the same space at the same time. Compartmentalization of transcription and translation. DNA = deoxyribonucleic acid RNA = ribonucleic acid Proteins are composed of 20 a.a., polymers of a.a. DNA and RNA - composed of 4 different nucleotides, polymers of nucleotides Nucleotide = nitrogenous base + 5C sugar + phosphate Deoxyribonucleotide = thymine cytosine adenine guanine Ribonucleotide = uracil cytosine adenine guanine Nomenclature for Nucleic Acids - DNA and RNA + deoxyribose + ribose + phosphate(s) + phosphate(s) deoxyribonucleoside ribonucleoside Structure of a nucleotide Two types of nitrogenous bases 5 5 2 6 2 6 4 4 hydrophobic Building blocks of DNA Building blocks of RNA Soluble in water
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