name two factors involved in differentiating vetnral endoderm into liver lineages
FGF-from the cardiac mesoderm BMP- from the septum transversum mesenchyme cells -induce liver program and suppress the pancreas program
what are two transcription factors that help regionalize the gut?
Pdx1 and Hex--DNA binding proteins that activate transcriptional programs, regionalized patterns of expression in different parts of the digestive system
how might you show that Hex expression marks the birth of the liver?
hepatoblasts are stained blue by marking Hex, you can look at different time points to see the stages of liver bud organogenesis, Hex expression correlates to the budding/birth of the liver, can see it move out and develop