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When is a plant cell plasmolyzed?
What is a hypertonic solution?
What are some of the functions of cell membranes?
Four parts of a spectrophotometer
Standard curve limitations
Aspetic techniques that can be used
What does the blank used with a spectrophotometer consist of?
How is a spectrophotometer zeroed?
Define each term in the "fluid mosaic model"
Explain phospholipid bilayers
How are bacterial species identified?
What is the best way to isolate individual cells?
Steps of a gram stain
Commonly reocognized cell morphologies
What are three other ways to identify bacteria besides morphology?
How is motility in bacteria tested?
How is formation of endospores determined in bacteria?
What are the different enzymatic activities in bacteria that can be tested?
How can resolution be decreased (improved)?
How is the resolution value calculated?
Why do Gram-positive bacteria stain purple?
Why do Gram-negative bacteria stain pink?
What is the effect of high temperatures on bacteria that do not form endospores?
How do microfilaments act in amoeboid movement?
Phase contrast microscope
How can contrast be improved?
What effect would cytochalasin (inhibits microtubules) and cholchicine (inhibits microfilaments) have on Pelomyxa?
What effect would cytochalasin (inhibits microtubules) and cholchicine (inhibits microfilaments) have on Euglena?
What effect would cytochalasin (inhibits microtubules) and cholchicine (inhibits microfilaments) have on motile prokaryotes?
What is phagocytosis, and how does it differ from receptor-mediated endocytosis?
What are enzymes made up of?
What is an active site?
What series of reactions occurs in photosynthesis?
What were the controls in the photosynthesis in spinach chloroplasts experiment?
What are the independent and dependent variables for the absorbance spectrum of photosynthesis in spinach chloroplasts?
What are the independent and dependent variables for the action spectrum of photosynthesis in spinach chloroplasts?
Why are spinach leaves green?
At what wavelength is the action spectrum measured at?
Where do light reactions take place in the chloroplast? Reactions of the Calvin cycle?
How is ATP produced?
Glycolysis in eukaryotes
After glycolysis, what occurs in the presence of oxygen?
After glycolysis, what occurs in the absence of oxygen?
What sort of feedback system occurs in alcohol fermentation?
Physiological response curve
Why is fermentation necessary?
Why was the yeast flask swirled prior to adding yeast to each tube?
What would happen if the metabolism in yeast experiment were done without the 10 minute pre-incubation period?
What process are the yeast in the Durham tube undergoing?
What metabolic processes occur in the cytoplasm?
What metabolic processes occur in the mitochondria?
Bacterial genomic DNA
Bacterial plasmid DNA
How can high molecular weight (HMW) DNA be extracted?
What does centrifuging do?
What does vortexing do?
Why is STE added to the DNA treatment?
What is Solution I in the DNA treatment?
What is Solution II in the DNA treatment?
What is Solution III in the DNA treatment?
What does centrifuging do to the genomic DNA-potassium-SDS-protein-cell wall complex?
What does the 95% ethanol wash do in the DNA treatment?
What does the 70% ethanol wash do in the DNA treatment?
Why is the 30 minute incubation period necessary in the DNA extraction?
Why is sodium acetate used to precipitate HMW DNA?
What are the 2 properties of DNA that allow you to separate genomic DNA from plasmid DNA
How can HMW DNA be extracted from solution?
What would happen if the tube were vortexed after the addition of Solution II?
What is the difference between genomic DNA, plasmid DNA, and eukaryotic DNA?
What is the purpose of the 95% ethanol and 70% ethanol wash?
Why must plasmid DNA be kept on ice following incubation?
What was the experiment performed by Avery, MacLeod, and McCarty?
How did the experiment performed in Biol 107 differ from Avery et al?
How were kanamycin sensitive E. coli cells made proficient to take up DNA?
How is a competent cell transformed?
What is kanamycin?
What occurs in kanamycin resistant cells?
Plate Count Method
Petroff-Hausser Counting Chamber
Optical Density (OD)
Why is Solution T (Tris) buffer used in the Transformation of Bacterial Cells lab?
What occurs during the first incubation period of the Transfomation of Bacterial Cells lab?
What occurs during the heat shock incubation period in the Transformation of Bacterial Cells lab?
What does the third incubation period in the Transformation of Bacterial Cells lab do?
What would occur if the environment in which the E. coli was grown was anaerobic?
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