it had been debated whether genes were made of proteins or DNA. Many believed they were made of proteins because proteins had a very complex structure, and DNA only had 4. But after the Hershey & Crick experiment, it was cocluded that genes were made of DNA
What was the Hershey & Crick experiment
used radioactive material to determine if genes were composed of proteins or DNA. They predicted that if genes were composed of DNA than the radioactive material would be found on the outside of the infected cell ghost capsids and the radioactive DNA would be found inside of the cell
What was the conclusion of the experiment?
genes were made out of DNA, the radioactive proteins were found in the ghost capsids and the radioactive DNA was found inside of the cell
what is the primary structure of DNA molecules?
Backbone made up of sugar and phosphate groups of DNA, they also have a series of nitrogen containing bases that project from the back bone
All strands of DNA have...
Directionality and polarity
What did Watson and Crick come up with?
The idea of antiparallel in which they would line up the two strands to the opposited ends of one another. They relized that therse strands would spiral areou one another creating a double helix
What is the Double helix
When strands of DNA helix around one another due to nitrogenous bases fitting in pairs within the spiral and forming hydrogen bonds
Complementary Base pairing
Secondary structure stabalized by the H bonds that form between Adenine, Thymine, and the bases of guanine, and cytosine
Polymerizes deoxyribonuclotides to DNA, this protein catalyzes the synthesis of DNA *DNA polymerase can only add to the 3' side of the growing DNA chain*
In what direction does DNA synthesis occur
Why are reactions in cells exergonic?
the monomers act as substrates are doxyribonucleotide Triphosphates (dNTP) (N=base in DNA)
How is replication started?
Replication bubbles form @ specific sequence of bases(orgin of replication)
Replication bubbles grow as DNA replication continues because synthesis is bidirectional
Y shaped region where parent DNA double helix splits into 2 single strands- they are then copied
How is the helix opened and stabilized
Want to see the other 16 Flashcards in Chapter 14?JOIN TODAY FOR FREE!