Florescence microscopy may be used to:
Determine spatial localization of specific proteins
Determine presence of mRNA
Determine presence of dna
Determine presence of a point mutation
A very thin plane of the section is seen in focus at one time.
Several focused planes of a given specimen are used to reconstruct a three dimensional image.
The objects appear lighter and darker relative to each other.
Used to view living cells.
Tissue sections are irradiated with UV light of a given wavelength and the emission is in the visible portion of the spectrum.
Direct and indirect fluorescent techniques used to identify the spatial location of specific proteins in tissues.
Shows only surface views.
Inside of organs are viewed by freezing and fracturing tissues to expose their internal surfaces.
1. Anastamosing network of interconnecting channels and sacs formed by a continuous membrane which encloses a space.
2. Generation of proteins
Endoderm cells become:
Lining cells of the stomach
Epithelial cells of the lungs
All of the above
Mesoderm cells become:
All of the above
1. Cell synthesizes and secretes components of BL
2. Polymerization outside the cell.
3. Laminin synthesized by epi. cells polymerizes at the basal surface, initiates the formation of BL
4.The laminin polymers are anchored by integrins.
5.Type IV collagen is synthesized by fibroblasts and form suprastructure close to laminin polymer
6.These networks are connected by the entactin or nidogen
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