- Temple University School of Podiatric Medicine
- Histology 101
- Exam 1
Last Modified: 2014-09-16
Florescence microscopy may be used to:
Determine spatial localization of specific proteins
Determine presence of mRNA
Determine presence of dna
Determine presence of a point mutation
A very thin plane of the section is seen in focus at one time.
Several focused planes of a given specimen are used to reconstruct a three dimensional image.
The objects appear lighter and darker relative to each other.
Used to view living cells.
Tissue sections are irradiated with UV light of a given wavelength and the emission is in the visible portion of the spectrum.
Direct and indirect fluorescent techniques used to identify the spatial location of specific proteins in tissues.
Shows only surface views.
Inside of organs are viewed by freezing and fracturing tissues to expose their internal surfaces.
1. Anastamosing network of interconnecting channels and sacs formed by a continuous membrane which encloses a space.
2. Generation of proteins
Endoderm cells become:
Lining cells of the stomach
Epithelial cells of the lungs
All of the above
Mesoderm cells become:
All of the above
2. Where are they found
2. Ducts, kidney tubule
- Create functional barrier
- Keep protein in the correct area
- Regulate transport down electroosmotic gradients
- Separate apical plasma mem. domain form basal and laeral domain.
- Allows for specialization and recognition of diff. molecular signals
- Regulation of passage of solutes between cells
- Cell to cell adhesion
- Cell to cell communication
4. Function (2)
5. Main protein associated
2. Above zonula adherens
4. Regulates selective passage of solutes, prevents diffusion of apical and basolateral transmembrane proteins
5. Claudins- transmembrane proteins
1. Cell synthesizes and secretes components of BL
2. Polymerization outside the cell.
3. Laminin synthesized by epi. cells polymerizes at the basal surface, initiates the formation of BL
4.The laminin polymers are anchored by integrins.
5.Type IV collagen is synthesized by fibroblasts and form suprastructure close to laminin polymer
6.These networks are connected by the entactin or nidogen
- Link btw. actin cytoskeleton and the ECM proteins via integrins
- Sense mechanical changes in the ECF (contraction), convert them to biochemical signals and transmit them to cells.
- Maintain the integrity of the epithelium-CT tissue interface.
Words From Our Students
"StudyBlue is great for studying. I love the study guides, flashcards, and quizzes. So extremely helpful for all of my classes!"
Alice, Arizona State University
"I'm a student using StudyBlue, and I can 100% say that it helps me so much. Study materials for almost every subject in school are available in StudyBlue. It is so helpful for my education!"
Tim, University of Florida
"StudyBlue provides way more features than other studying apps, and thus allows me to learn very quickly! I actually feel much more comfortable taking my exams after I study with this app. It's amazing!"
Jennifer, Rutgers University
"I love flashcards but carrying around physical flashcards is cumbersome and simply outdated. StudyBlue is exactly what I was looking for!"