"You can never turly know what's going on but can bring in other approaches in attempt to figure it out"
traditional biochemical view of the cell (1960's)
cell is a bag of enzymes based on the observation that complex chemical reactions could occur with materials obtained from ground up cells
the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye (objects not within the resolution range of the normal eye). Well-known branches: optical, electron, and scanning probe microscopy.
ability of a microscope to identify 2 objects separated by distance x as distinct
key limit resolution
minimum distance is the wave length of source of illumination (light microscopy)
theoretical limit of resolution
minimum distance is half the wavelength of source illumination, usually ~250nm