Exam 2 (Chapters 6)
Last Modified: 2016-03-08
Formula: No Crossover
Formula: Crossing over between outside loci
Formula: No crossing over between outside loci
Map distance must take into account all crossovers (even silent ones)
take into consideration that some multiple crossovers will be undetected among the offspring
r= distance between loci/100
Probability of event occurring vs. probability of a specific phenotype resulting (DCO)
The probability of the event ocurring would be the regular formula
The probability of a specific phenotype resulting would be the regular formula divided by 2 DCO=(1/2)r1r2C
How to determine NCOs when making a linkage map
paired gametes with the largest total
paired gametes with smallest total
paired gametes with intermediate totals
How to determine gene order
compare one NCO and one DCO with two shared phenotypes. The phenotype which differs between these is the middle locus; F1 will have NCOs on top and bottom of DNA strand
Formula: Calculating mapping distances
#DCOs+#SCOs (in common with the crossover between 2 genes)/TOTAL=r; then multiply by 100
C=Observed DCOs/Expected DCOs = #DCOs/(r1xr2xtotal)
cM can be thought of as
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