provide three reason why the use of aseptic technique is essential when handling microbial cultures in the laboratory
aseptic technique insures that no contaminating organisms are introduced into culture materials, insures that organisms do not contaminate to the handlers or others, and lastly insures that no contamination remains after you have worked with cultures
provide two examples of how the Bunsen burner is used during inoculation of a tube culture
to burn the loop and the mouth of the tube before inoculation, and then to burn the loop and the mouth of the tube after inoculation
How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample to/from an agar plate?
by practicing aseptic technique of burning the loop before introducing the loop to the plate, also by keeping the lid of the agar plate closed when not in use, and by opening the lid as minimally as possible and for as short of time as possible
Where should a label be written on an agar plate?
On the bottom, media, side of the plate, so the label is easily seen. Also the lid may get lost from the media and then no one knows what is growing in the media
How should agar plates be incubated? Why?
Inoculated plates should be incubated upside down. This prevents moisture from condensing on the agar surface and spreading the inoculated organisms.
Against which organisms are disinfectants effective? Against which type of organisms may they not be effective? What disinfectant(s) is used in your laboratory?
disinfectants are effective against vegetative cells and viruses, but may not be effective in destroying endospores