Extracellular Enzymatic Activity And Carb Fermentation Ex 21 22
What is the principle of extracellular enzymatic activites of microorganimsm?
Polysaccharides like lipids and starches are high molecular cannot permeat cell membrane. So, they are hydrolyzed by specific extracellular enzymes into basic building blocks. The polysaccharides then become low molecular and used for the synthesis of protoplasmic requirements and energy production.
High molecular weight branching polymer of glucose by glycosidic bonds. Its hydrolyzed amylase which is extraceullar enzyme and becomes small branch called dextrin and turns into maltose. Then its hydrolyzed by maltase and turns into a low molecular weight glucose
Whats the purpose of starch agar? What is it composed of?
Demonstrate hydrolytic activities of exoenzymes. Nutrient agar supplemented with starch. Will have a clear zone around the microbe that can hydrolyze starch. It will be negative if e starch cannot split.
Degradation of lipids like triglycerides is split by extracellular enzyme lipase(esterases). Ot cleaves ester bonds by the addition pf water to form the building block glycerol (alchy) and fatty acid.
Oknce the small lipid is in the cell, ot can be further metabolized through which pathway?
Aerobis respiration to produce cell energy Atp. May enter pathway for synthesis of protoplasmic requirements.
Whats the purpos epf trybutyrin agar? What is it composed of? Tributyrin forms what?
Demonstrate hydrolytic activities of exoenzyme lipase. Composed of nutrient agar supplemented with triglyceride trybutyrin as the lipid substrate. Forms an emulsion when dispersed in agar, producing an opaque medium that is neccessary for observing oxoenzymatic activity.