contaminated food/water leading to Hepatitis A and E.
Identify the main health concern associated with viral hepatitis
main concern is with chronic infection because it could possibly lead to cirrhosis and/or liver
recognize the significance of IgM isotope antibody, IgG isotope antibody, and viral Ag or nucleic acid(RNA or DNA) in general staging of viral infections by laboratory testing
Virus-specific antibodies in adult serum,
IgM isotope indicates a current or recent acute infection. IgG isotope indicates a current or past infection and immunity(in most cases)
Virus-specific IgM isotype in newborn serum indicates congenital infection(IgG does NOT because it could be from mom)
Positive serologic tests for viral Ag or detection of viral DNA or RNA indicates current infection.
Explain how a RIBA test (such as that used to confirm HCV infection differs from a western blot test
RIBA= Recombinant Immunoblot Assay
it is a confirmatory test and a molecular assay for HCV RNA in patient's serum. It is similar to western blot test, except recombinant HCV proteins are blotted directly onto the nitrocellulose membranes. The western blot is the confirmatory test used, and it uses 10 borrella proteins transferred to nitrocellulose and blotted with patient serum
Given the results of "hepatitis panel" tests for acute viral hepatitis interpret the disease that the patient has and suggest whether further testing is warranted and what the testing should be.
When a patient presents with jaundice or other signs of acute hepatitis a panel is ordered for the 3 most important forms of acute infection in the US(Hep A, Hep B, Hep C) the ordered test is:
-IgM anti-HBc and HBsAg
-anti-HCV and HCV RNA
If results for IgM anti-HBc and HBsAG or anti-HCV and HCV RNA come back poitive: we want to stage the infection to see if they will have immunse response to the infection. Panel results should help determine if hepatitis is due to viral infection or not and what virus.