Last Modified: 2011-07-07
- Bright field
- Dark field
- Phase contrast
Sample usually requires contrast
Objects reflect light; appear larger & brighter
Light is defracted by objects; increases their contrast
Background is black
objects that absorb and emit light at longer wavelengths are visible
Fluorescent compounds can be added to samples (that cant be seen with other microsopes) in order to make them visible
Provides for both higher magnification and resolution
Transmission electron microscope
very high magnification
Higher than SEM magnification
The "e" appears upside down
The "e" moves to the left
A. Choose INANIMATE object to screen for the presence of microbes
B. Remove sterile swab
C. Dip water & rub on area being tested
D. rub swab on media
E. Place lid back on plate & label dish
DON’T SWAB BODY PARTS!!!!!!!
What factors affect #s & types of microbes in air? air source, wind, temperature, animals/ plants
Discuss where u want to test
Label BOTTOM of dish as directed
Place dish n desired location
Remove lid & return 2 class
contains agar- solidifying agent
incubated at 37 to allow night growth
Microbes have global distribution and some are found in practically all
What two precautions are necessary in lab because of the ubiquity of
using aseptic technique in all transfers and minimizing exposure of
cultures to prevent contamination
List two benefits derived from the microbes that live within and upon our
How can different species of bacteria be distinguished by examining their
Is the amount of growth on the agar plate an exact representation of the
microbes present in the environment sampled? Explain
no, probably some microbes present that are unable to grow on growth medium
Which area(s) appeared to contain the greatest number of microbes?
table and mouth
- with time surfaces accumulate many microbes
- and mouth provides warmth, nutrients and moisture for microbial growth
What temperature would be the optimum incubation temperature for those microbes
isolated from the mouth.
prevents moisture from accumulating on the top, and water dripping on
microbial growths spreading them on surface
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