Lab 18: Evaluation of Antiseptics: Filter Paper Disk Method
Last Modified: 2016-03-08
- take a few loopfuls of bacteria and add to hot agar to create a stock solution
- distribute solution in plates
- dunk 1/2 filter paper square into antiseptic solution and add to plate after agar solidifies
- species of organism used (some are more susceptible to different antiseptics than others)
- concentration of antiseptic
- solubility of antiseptic (how fast it can travel through the agar)
- size of the molecule (again, how fast it can travel. larger molecules will be slower)
- charge of molecule ( charge interactions with agar may slow it down)
- toxicity towards organism (see first point)
- potency of an antiseptic as compared to phenol
- calculated by comparing the zones of inhibition of phenol and test compound
- assumes that the test compound has the same molecular properties (diffuses at the same rate) as phenol
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