no requirement for ultra-thin serial section reconstruction
an example is CAT
The electrons interact with the atoms that make up the sample producing signals that contain information about the sample's surface topography, composition, and other properties such as electrical conductivity.
proteins are separated in the electrophoresis by size (not shape, or charge)
gel electrophoresis to separate proteins by 3-D structure or denatured proteins by the length
protein transferred to a membrane and detected using antibodies specific to the target protein
used by molecular biology research to study gene expression
developed by Edwin Southern
the principal that molecules dissolved in a solution will interact with a solid surface
ion-exchange chromatography, and gel filtration chromatography, affinity chromatography
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