Affinsen Experiment Scrambled ribonuclease - Traces of Beta Native ribonuclease Traces of beta mercaptoethanol unscrambles it Did he prove there is no such thing as a chaperone? It depends how big the protein is HPS-70 is the heat protein shock, that helps for RNA folding, PH Buffer. CH3 CH3 Ch3 | | | H3N?C?CooH PKa= 2.3 NH3?C?COO PKa=9.9 H2N?C?COO -1 0 +1 Ph2 Ph=7 pH=12 Isoelectric point-the average of the 2 surrounding pkaS pI=pka1+Pka2/2 =2.3+9.9/2=6.1 This whole system is fragile and can move either way by the addition of solvents Henderson Hasselbach- =pH=pka+log [A]/[HA] Test question= you have 1 mole of alanine at its Isoelectic point. We add .3 moles of HCL, what is the new pH? HCL vs NaOH A+HA= alanine 7+3=10 Pka=2.3 Ph=2.3 + log(7/3) and the log of 1=0 And log of .5= to a ? number So if you add .1 mol of NaOH then the number would be negative Pka=9.9 pH=9.9+log (1/9)
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