MCDB 306: GENETICS LABORATORY Fall 2009 Schedule Date Experiment September 14-15 1: Introduction to Drosophila Use of micropipettors September 21-22 1: Introduction to Drosophila project Mapping “unknown” mutations in Drosophila 3: Microbial techniques—growth curve; replica plating September 28-29 1: Drosophila—patterns of inheritance Start all initial crosses October 5-6 1: Assignment of genes to chromosomes 4: Start conjugation of E. coli October 12-13 1: Analysis of F1 progeny Starting second series of crosses 4: Completion of conjugation October 19-20 FALL STUDY BREAK 1: Remove parents from the cross bottles October 26-27 1: Analysis of F2 and backcross progeny Final mapping of unknown mutations Completion of Drosophila analysis and skeletal report November 2-3 EXAM I 2: Introduction to Caenorhabditis elegans Making first set of crosses November 9-10 2: C. elegans – creating mutant/marker heterozygotes 6: DNA fingerprinting using a VNTR locus Analysis of human DNA by PCR (Part I) November 16-17 2: C. elegans—isolation of F1 hermaphrodites 6: DNA fingerprinting (Part II) Human Population Genetics November 24 – 25 THANKSGIVING WEEK 2: C. elegans—lysis of F2 mutant progeny Heat treatment and multiplex PCR 7: Bioinformatics—assign projects November 30-December 1 2: C. elegans—gel electrophoresis of PCR products 7: Bioinformatics—students’ presentations December 7-8 EXAM II MCDB 306: GENETICS LABORATORY Course Grade Distribution 30% of grade............ Lab Reports Genetic analysis and mapping in Drosophila - Skeletal Report (15%) Drosophila Lab Report (15%) 40% of grade............ Midterm and Final Exam Two exams will be given during laboratory periods. 27% of grade............ Notebook, Homework and Lab Write-Ups You are required to keep a lab notebook for this course, which will be checked occasionally during the semester. Your lab notebook should be a complete and accurate record of your observations, results and analysis of results. It should contain: experiment protocols, your data, experimental methods not outlined in the lab manual, deviations from the procedure, mistakes or problems encountered in the lab, and tables and charts. These 27% of the course grade will be distributed between four major assignments and additional homework. Conjugation in E. coli Lab Report (12%) Genetic Analysis of a C. elegans mutant Lab Report (6%) VNTR assignment (3%) Bioinformatics assignment (3%) Additional Homework assignments (3%) 3% of grade............ Check-offs and Attendance You must show your instructor the final results of all the experiments in microbial genetics in the form of bacterial or yeast plates. You are expected to attend every lab. Inform your GSI before any planned absence (interviews, etc.). If you do miss a lab or lecture, be sure to check with your GSI immediately.