- University of Tennessee - Knoxville
- Microbiology 310
- Microbial Nutrition
Last Modified: 2011-07-02
Related Textbooks:Microbiology: An Evolving Science (Second Edition)
-source of this often supplies hydrogen, oxygen and electrons
-must obtain energy from other sources
-nitrate via assimilatory nitrate reduction
-nitrogen gas via nitrogen fixation
-essential cell components that the cell cannot synthesize
-must be supplied by the environment of cell is to survive and reproduce
-purines and pyrimidines (needed for nucleic acid synthesis)
-vitamins (function as enzyme cofactors)
-industrial production of growth of factors by MOs
-direction is from area of high concentration to low concentration but uses carrier molecules the transport
-need less of a gradient than passive dif.
MORE PROMINENT IN EUKARYOTES THAN IN PROKARYOTES
-moves molecules AGAINST the gradient
-concentrates molecules inside the cell
-involves carrier proteins (permeases)
-seen in bacteria, archaea, and eukaryotes
-move molecules across the membrane with aid of ATP hydrolysis
-energy dependent process
-transports sugars while phosphorylating them using phopshoenolpyruvate (PEP) as the phosphate donor
-microorganisms use siderophores to aid uptake
-siderophore complexes with ferric iron
-complex is then transported into the cell
EXTRACTS: aqueous extracts, usually of beef or yeast
AGAR: sulfated polysaccharide used to solidify liquid media
-diluted samples mixed with liquid agar
-mixture of cells and agar are poured into sterile culture dishes
-slowest at center of colony
-in nature, many microorganisms form biofilms on surfaces
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