Module 2 - Part 3 - Microbiology, Immunology, And Molecular Genetics 500 with Booton at The Ohio State University

Chelsea G.

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Splicing of eukaryotic pre-mRNA involves ___ reactions and occurs in the ___ .

transesterification

nucleus

Heinz Shuster collected the following data on the base composition of ribgrass virus

On the basis of this information (see below) is the hereditary information of the ribgrass virus RNA or DNA? Is it likely to be single stranded or double stranded?

RNA

single stranded

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5' ...GGAGCUCGUUGUAUU... 3'

is this sequence DNA or RNA? how can you tell?

RNA because U is present

While actually a form of RNA polymerase, this enzyme lays down the initial nucleotides to set up a condition where DNA polymerase can then take over for replication.

primase

Match numbers with the best letter choice:

continuous synthesis

leading strand

Match numbers with the best letter choice:

DNA primase

RNA primer synthesis

What would Avery, Macleod, and McCarty have concluded if their results had been that only RNAse treatment of the heat-killed bacteria prevented transformation of genetic virulence?

that RNA was the genetic material

A bacterial protein is encoded by the following mRNA sequence: AUGGUGCUCAUGCCCTAA.… The second methionine codon (AUG) in this mRNA sequence will

encode unformylated methionine.

Hairpins are formed in DNA as a result of

sequences on the same strand that are inverted and complementary

A friend gave you the following molecule and told you that it was a fragment of his RNA. Do you believe him? Justify your answer.

5’ TCC TGA CGA TGC TAC CGA 3’

No, the molecule has T’s. If it were truly RNA, it would have U’s rather than T’s

A segment of DNA from Drosophila melanogaster has the sequence:

5’ TCA AGC TTA AGA AGG CAT TTT 3’

if this sequence is the coding strand, what is the sequence of the encoded mRNA

5’ UCA AGC UUA AGA AGG CAU UUU 3’

A segment of DNA from Drosophila melanogaster has the sequence:

5’ TCA AGC TTA AGA AGG CAT TTT 3’

Assuming that this is the template strand, what is the sequence of the encoded mRNA

5’ AAA AUG CCU UCU UAA GCU UGA 3’

Which of the following statements best describes the messenger RNA?

mRNA is the only type of RNA that carries DNA's protein building instructions

The spliceosome is a large enzyme that catalyzes removal of specific

introns in pre-mRNAs

The anticodon on the tRNA molecule:

binds to the mRNA in a complementary fashion

Heterochromatin is characterized by all of the following, EXCEPT that it

contains genes that require high levels of transcription

In eukaryotes, tRNAs are transcribed ________ and function ________

in the nucleus;

in the cytoplasm

In what cellular compartment are introns removed from pre-mRNA to make mature mRNA?

nucleus

Which one of the following statements regarding eukaryotic transcriptions is not true?

A group of genes is transcribed into a polycistronic RNA.

Two eukaryotic proteins were found to be very similar except for one domain that was very different. Which of the following processes is most likely to have contributed to this phenomenon?

differences in pre-mRNA splicing that results in an altered pattern of exon inclusion.

Match numbers with the best letter choice:

DNA ligase

phosphodiester bonds at DNA nicks

What does “proofreading” refer to with regard to DNA replication

ability for dna polymerase to remove and replace incorrectly paired nucleotides in the process of replication

This term describes the sequence of nucleotides which direct the formation of a new nucleic acid strand.

template strand

Here is a list of eukaryotic RNA polymerases. Match them with what they transcribe.

RNA Pol I; RNA Pol II; RNA Pol III

a. mRNA b. tRNA c. rRNA d. snRNA

RNA Pol I: a. mRNA
RNA Pol II: a. mRNA, and d. snRNA
RNA Pol III: a.mRNA, c.rRNA, and d.snRNA

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