November 6, 2008 BIOL 203 Notes Random Match Probability What is the likelihood the suspect will match the genotype of the sample by random chance? Depends on how rare the alleles are in a population We want to know the locus frequency How freq. will you find the combination of allelesgenotype Formula for finding this Why this formula? Hardy-Weinberg principle Can evaluate populations looking for evidence of evolution p²:2pq:q² The power of Discrimination increases with the number of loci profiled Virtual lab?dideoxy sequencing Dideoxy Sequencing Given an unknown sequence, we can find what that sequence is by synthesizing more and more of it from a few nucleotides to the whole length Identify the length and order of that sequence Then identify where each of the fragments end atthe final nucleotide in the fragment Chain-terminating analogs ddNTP they need a primer to get started synthesis is always going to start at the same place! Make the primer radioactively labeled Gel from bottom up5? to 3? Smallest one will be 5? end Don?t go beyond first unknown bact. Sample in virtual lab One of the links goes to an NCBI website which talks a lot more about the statistics?.not necessary to study Stuff for this week!!!
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