-pyruvate to acetyl coA -requires input of coenzyme A -liberates CO2 -commits cell to TCA cycle -drives metabolism towards electron transport chain -forms reducing power 2NADH + 2H+ -form 1 precursor metabolite -occurs 2x because of 2 pyruvate produced in glycolysis
-also called krebs or citric acid cycle -used in aerobic and anaerobic -8 step cycle that converts acetyl co A into oxaloacetate -produces = 4CO2, 2 ATP, 6NADH + 6H+, 2 FADH2 -forms 3 precursor molecules
major functions of electron transport chain:
-reoxidation of reduced coenzymes (NADH and FADH2) critical for recycling -generation of ATP via oxidative phosphorylation
electron motive force relies on what?
electron gradient (step wise reduction in potential energy) -stepwise release of energy creates a proton motive force -proton motive force used to drive ATP synthesis
use SIM agar deep -use Kovacs reagent to top and if red color then (+) -black = H2S -motility = movement away from stab
methyl red and voges proskauer:
-use MRVP broth (buffered peptone-glucose) -used to distinguish between 2 classes of fermentation (mixed acid and 2,3-butanediol) -Methly red test = mixed acid (red color +) -voges test = 2,3 butane. (rose color = +)
-use Simmon's Citrate agar -test ability to use Citrate as sole C source -must have citrate permease to TRANSPORT and then citrase to OXIDIZE once inside cell -pH indicator bromthymol blue (+ = blue/- = green)
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