- University of California - Los Angeles
- Life Sciences
- Life Sciences 3
Last Modified: 2012-03-21
Catalyzed the peptide bond formation
2. decoding the codon
3. Help the peptide bond formation
D-loop contains two dihydrouridine
T-Loop - contains pseudouridine
Variable loop - varies 4-13nt
Anticodonloop - bases of anticodon flip out and often contain inosine (for wobble pairing)
If the radioactive maino acid is recognized by and attached to the correct tRNA, it was radioacitvely labeled.
20 sets of 64 reactions required to break genetic code.
Which amino acids are degenerated?
Which are not?
Arginine, Leucine, Serine are all degenerated the most
Methioinine and Tryptophan are not degenerated
(Methionine is the first one)
Mismatching U with A or G
or G with U or C
Or Inosine, can base pair with A, C and U
ARS proofreads and remove mistakenly incorporated residues
Using ATP, ARS covalently attaches amino acid to the 5' phosphate group of AMP
ARS transfers amino acid from AMP to 3' -OH group of tRNA
Can have multiple SDS, all work independently
Initiation factors (1) prevent two free ribosome subunits from binding to each other and (2) help assemble 30S initiation complex.
Always starts with formylated methionine
eIF4E - binds to cap
eIF4G - adaptor protein can bind to many other proteins, and binds to polyA tail, creating circular mRNA
eIF4A - RNA helicase activity
eIF4H - stimulates eIF4A helicase cativity
When insulin is present, it activates a kinase called mTOR. mTOR phosphorylates PHAS-1 which does not bind to eIF4E. Free eIF4E binds to cap of mRNA and activates initiation
When the oocyte is activated, a kinase called Eg2 phosphorylates CPEB which brings in CPSF (Poly adenylation specificity factor) and changes conformation of Maskin, releasing eIF4E. eIF4E binds to eIF4g to turn on translation.
The first amino acid arrives in P site, The first amino acid to attach to that enters A Site.
Peptide formation catalyzed by peptidyl transferase. Believed that 23S or 28S rRNA has catalytic properties.
New aminoacyl enters A site. Peptide bond is formed between A site and P site. The P site is now empty, and ribosome moves toward the 3' end of mRNA, and each tRNA moves over one.
Words From Our Students
"StudyBlue is great for studying. I love the study guides, flashcards, and quizzes. So extremely helpful for all of my classes!"
Alice, Arizona State University
"I'm a student using StudyBlue, and I can 100% say that it helps me so much. Study materials for almost every subject in school are available in StudyBlue. It is so helpful for my education!"
Tim, University of Florida
"StudyBlue provides way more features than other studying apps, and thus allows me to learn very quickly! I actually feel much more comfortable taking my exams after I study with this app. It's amazing!"
Jennifer, Rutgers University
"I love flashcards but carrying around physical flashcards is cumbersome and simply outdated. StudyBlue is exactly what I was looking for!"