Can use any fixative except --- with Feulgen reaction
Specimen thickness and preparation with feulgen reaction
routine paraffin, 4 microns
Feulgen Reaction Mode of action
Hydrolysis of DNA by hydrochloric acid
Feulgen reaction mechanism
hydrolysis is the removal of purine bases, adenine and guanine, from the sugars and phosphate groups. Hydrolysis caused by hydrochloric acid generates an aldehyde group that can be demonstrated with Schiff's reagent.